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Effect of SM on R and Z expression from a bicistronic R-Z gene transcribed from the CMV IE promoter. CMV-RZ, which contains a BRLF1 and BZLF1 bicistronic gene driven by the CMV IE promoter, was transfected into 293 cells either with empty vector (C/SM) or with SM expression plasmid (lanes 2 and 4). Protein lysates were made 48 h after transfection and analyzed by immunoblotting using anti-R antibody (A) anti-Z antibody (B), or antiactin antibody as a loading control (C).
