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. 2009 Jun 3;83(16):8270–8275. doi: 10.1128/JVI.00670-09

FIG. 3.

FIG. 3.

Biological function of feTRIM5. (A) Mouse fibroblasts which lack restriction activity were transduced with domestic feTRIM5 or huTRIM5α. While huTRIM5α specifically restricts N-tropic but not B-tropic MLV, feTRIM5, which lacks a B30.2 domain, restricts neither MLV-N nor MLV-B. Nor is feTRIM5 able to restrict lentiviral vectors derived from HIV-1 and simian immunodeficiency virus from rhesus macaques. MDTF, M. dunni tail fibroblasts. (B) feTRIM5 acts as a dominant negative agent against huTRIM5α-mediated restriction. The TE671 cell line, which expresses endogenous TRIM5α, was stably transduced with feTRIM5 or with huTRIM5 P306STOP, which bears a stop codon at the residue corresponding to that in feTRIM5. Expression of feTRIM5 or huTRIM5 P306STOP rescued MLV-N infectivity. Error bars represent mean ± standard error (n = 3).