FIG. 3.

Intracellular localization of NP and NF90 during influenza A virus infection. A549 cells were infected with VNM1194 at an MOI of 5. Infected cells were processed for microscopy at 4 and 10 h postinfection (h.p.i.). After fixation, the cells were stained with anti-NP and anti-NF90 monoclonal antibodies coupled with Zenon mouse IgG Alexa 488 and Alexa 594, respectively (Invitrogen). DAPI (4′,6′-diamidino-2-phenylindole) was used to visualize the nuclei of the cells. Imaging of cells was performed by using an LSM 510 Meta confocal laser scanning microscope (Carl Zeiss).