FIG. 6.

NF90 inhibits viral genome replication and gene expression. A549 cells were treated with NF90 specific or nonspecific siRNAs for 24 h, and cells were then infected with H5N1 virus (A/Vietnam/1194/04) at an MOI of 1, as described in Materials and Methods. NP and NS1 vRNA (A) and mRNA (B) levels in infected cells were estimated by quantitative RT-PCR at 4 and 8 h postinfection. (C) The expression of NP in virus-infected NF90 knockdown and control cells was compared by Western blot at 8 h postinfection.