FIG. 2.

NiV P residues 111 to 140 are critical for efficient inhibition of IFN signaling. NiV P WT and mutant constructs were cotransfected with an IFN-inducible, ISG54-driven firefly luciferase plasmid and a Renilla luciferase plasmid into 293T cells. NiV P mutant constructs include (A) internal deletions spanning residues 51 to 100 and (B) internal deletions spanning residues 101 to 150. At 24 hpt, cells were treated with IFN-β for 16 h. Induction levels are shown as a ratios of firefly-to-Renilla luciferase levels. Western blotting with anti-HA antibody for the NiV constructs is shown below each graph. IB, immunoblot.