FIG. 5.

The CARD-like domain and the mitochondrial membrane targeting sequence are essential for MAVS signaling. (A) Diagram illustrating the MAVS splicing variant and mutants. Mini, salmon MAVS splicing variant lacking residues 126 to 553; ΔCARD, salmon MAVS mutant lacking the CARD-like domain (residues 2 to 93); ΔTM, mutant lacking the mitochondrial membrane targeting sequence (residues 568 to 606). On the right is the size of each protein, in amino acids. (B) EPC cells were transfected with 2 μg of pcDNA vector encoding MAVS, mini-MAVS, and its mutants (ΔCARD and ΔTM). At 24 h posttransfection, the cells were infected with VHSV at an MOI of 5. The culture supernatants were collected at 48 h postinfection, and the viral titer was determined by plaque assay. The cell monolayers were stained with crystal violet at 5 days postinfection.