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Apparent Kms of C. glabrata Hst1 and Sir2. Relative HDAC activities of rHst1 (squares) and rSir2 (triangles) are shown. Bacterially expressed and purified Hst1 and Sir2 were incubated with [3H]acetylated histone H4 peptide (40,000 cpm/reaction mixture) in the presence of different concentrations of NAD+. The plots are from assays carried out in triplicate. The measured Km of rHst1 is approximately 118 ± 19 μM, and that of rSir2 is approximately 339 ± 40 μM.
