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Apparent Km values of Tna1, Tnr1, and Tnr2 for NA, NAM, and NRa
| Protein | Mean Km (μM) ± SEM
|
||
|---|---|---|---|
| NA | NAM | NR | |
| Tna1 | 0.59 ± 0.09 | 6.3 ± 1.7 | 110 ± 50 |
| Tnr1 | No uptake observed | 7.7 ± 1.2 | 1.6 ± 0.4 |
| Tnr2 | No uptake observed | 5.5 ± 1.1 | 1.5 ± 0.4 |
Uptake of radiolabeled precursors into a tna1 tnr1 tnr2 mutant strain (BG1442) expressing the gene for the indicated protein was measured.
