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. 2009 May 26;29(15):4220–4234. doi: 10.1128/MCB.01882-08

FIG. 1.

FIG. 1.

VPS75 interacts genetically with genes involved in transcription. (A) Scatter plot of the CCs of vps75Δ, asf1Δ, and rtt109Δ with all genetic profiles from the chromosome function E-MAP (9). The profile of RTT109 correlates with that of ASF1 (red) but not that of VPS75 (blue). (B) A subset of the genetic interaction profile for VPS75 as determined by E-MAP analysis, showing strongly interacting genes involved in transcription. Blue and yellow represent negative and positive genetic interactions, respectively. The DAmP (decreased abundance by mRNA perturbation) technique was used to generate hypomorphic alleles of essential genes (54). (C) VPS75 interacts genetically with a conditional allele of the gene encoding Rpb1, rpb1-104. Serial dilutions of the indicated strains were grown on YPD at 30°C for 2 days. (D) Deletion of VPS75 enhances the 6-AU sensitivity of a dst1Δ strain. Serial dilutions of the indicated strains were grown on medium lacking uracil (−U) or −U containing 50 μg/ml 6-AU (−U + 6-AU) at 30°C for 2 days.