FIG. 4.

Induction of GAL-VPS13 is affected by Vps75. Recruitment of RNAPII to the promoter and through the coding region (2.2 kb, 5.5 kb, and 8.3 kb) of GAL-VPS13 was measured by ChIP assays followed by quantitative PCR in wild-type (WT) and vps75Δ cells after 0, 30, and 60 min of induction. Values shown are the averages, with standard errors, for three independent experiments and are expressed as the amount of immunoprecipitated DNA corrected to input DNA. The value for the wild-type strain at 0 min was set to 1 and other values expressed relative to that. An unpaired t test was used to determine the significance of the difference between mutant and WT cells at each location after 60 min, with one asterisk denoting a P value of <0.05 and two asterisks denoting a P value of <0.01.