TABLE 1.
Saccharoymyces cerevisiae strains used in this study
| Name | Genotype | Source or reference |
|---|---|---|
| W303 1A | MATaleu2-3,112 his3-11,15 ade2-1 ura3-1 trp1-1 can1-1 | |
| JSY1099 | W303 1A VPS75-8His-2TEV-9Myc::URA3 | 59 |
| JSY1082 | W303 1A 8His-2TEV-9Myc-VPS75::URA3 | This study |
| JSY1083 | W303 1A 8His-2TEV-9Myc-VPS75::URA3 rtt109Δ::HIS3 | This study |
| JSY1076 | W303 1A vps75Δ::HIS3 | 59 |
| PHY2193 | MATahis3-Δ200 leu2-3,112 rpb1Δ187::HIS3 ura3-52 (pC6; rpb1-104 and pRS416) | 21 |
| JSY1101 | MATahis3-Δ200 leu2-3,112 rpb1Δ187::HIS3 ura3-52 (pC6; rpb1-104 and pRS416) vps75Δ::TRP1 | This study |
| JSY110 | W303 1A dst1Δ::HIS3 | J. Fellows |
| JSY1086 | W303 1A dst1Δ::HIS3 vps75Δ::TRP | This study |
| JSY1102 | W303 1A rtt109Δ::HIS3 | This study |
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | 71 |
| BY4741 vps75Δ | BY4741 vps75Δ::KANMX4 | 71 |
| BY4741 haa1Δ | BY4741 haa1Δ::KANMX4 | 71 |
| BY4741 vps75Δhaa1Δ | BY4741 haa1Δ::KANMX4 vps75Δ::URA3 | This study |
| FY2452 | MATaura3-52 his3 RPB3-HA1::LEU2 KANMX6-PGAL1-FLO8-HIS3 | 44 |
| FY2445 | MATaura3-52 his3 RPB3-HA1::LEU2 spt2::KANMX6 KANMX6-PGAL1-FLO8-HIS3 SPT6-FLAG | 44 |
| JSY1100 | MATaura3-52 his3 RPB3-HA1::LEU2 KANMX6-PGAL1-FLO8-HIS3 vps75Δ::URA3 | This study |
| JSY1103 | MATaura3-52 his3 RPB3-HA1::LEU2 KANMX6-PGAL1-FLO8-HIS3 rtt109Δ::URA3 | This study |