TABLE 3.
Genes differentially expressed between vps75Δ and wild-type yeast cells (change of ≥2.5-fold)
| ORF | Gene | Fold change in expressiona
|
Description | |||
|---|---|---|---|---|---|---|
| Expt 1 | Expt 2 | Expt 3 | Avg | |||
| Upregulated | ||||||
| YCR021C | HSP30 | 24.8 | 24.2 | 4.1 | 17.7 | Plasma membrane protein induced by various stresses |
| YDL037C | BSC1 | 21.7 | 13.2 | 2.4 | 12.4 | Transcript shows a high level of stop codon bypass |
| YGR249W | MGA1 | 13.5 | 15.4 | 2.1 | 10.3 | Similar to heat shock transcription factor |
| YDL038C | 13.8 | 13.1 | 3.1 | 10.0 | Putative protein of unknown function | |
| YGR052W | FMP48 | 9.2 | 9.2 | 2.4 | 6.9 | Unknown; localizes to mitochondria |
| YDL039C | PRM7 | 6.5 | 8.6 | 3.0 | 6.0 | Pheromone-regulated membrane protein |
| YPL014W | 6.6 | 7.7 | 3.2 | 5.8 | Putative protein of unknown function | |
| YGR138C | TPO2 | 5.8 | 6.1 | 3.3 | 5.1 | Polyamine transport protein of the major facilitator superfamily |
| YPR157W | 5.2 | 5.3 | 3.6 | 4.7 | Putative protein of unknown function | |
| YDL048C | STP4 | 3.9 | 4.5 | 3.1 | 3.8 | Kruppel-type zinc-finger-domain-containing protein |
| YER150W | SPI1 | 4.5 | 5.7 | 0.8 | 3.7 | GPI-anchored cell wall protein involved in weak acid resistance |
| YOR107W | RGS2 | 3.6 | 4.3 | 3.1 | 3.7 | Regulator of G-protein signaling for Gpa2 |
| YLR297W | 4.0 | 3.3 | 3.0 | 3.4 | Putative protein of unknown function | |
| YOL016C | CMK2 | 4.7 | 4.2 | 1.2 | 3.4 | Calmodulin-dependent protein kinase |
| YMR316W | DIA1 | 4.3 | 4.2 | 1.6 | 3.4 | Unknown, involved in invasive and pseudohyphal growth |
| YMR081C | ISF1 | 3.4 | 4.3 | 1.0 | 2.9 | Serine-rich, hydrophilic protein with similarity to Mbr1p |
| YOL014W | 3.4 | 3.5 | 1.0 | 2.7 | Putative protein of unknown function | |
| YER130C | 3.1 | 2.8 | 2.0 | 2.6 | Putative protein of unknown function | |
| YOR306C | MCH5 | 3.0 | 2.8 | 1.8 | 2.5 | Plasma membrane riboflavin transporter |
| YNL065W | AQR1 | 2.7 | 2.7 | 2.2 | 2.5 | Multidrug transporter of the major facilitator superfamily |
| YER037W | PHM8 | 2.7 | 2.8 | 2.0 | 2.5 | Unknown, possibly involved in phosphate metabolism |
| YOR032C | HMS1 | 3.3 | 2.8 | 1.4 | 2.5 | Basic helix-loop-helix protein |
| Downregulated | ||||||
| YHL048W | COS8 | −1.8 | −1.6 | −4.2 | −2.5 | Nuclear membrane protein, member of the DUP380 subfamily |
| YJR079W | −2.4 | −3.1 | −2.1 | −2.5 | Putative protein of unknown function | |
| YLR327C | TMA10 | −2.7 | −2.6 | −2.2 | −2.5 | Unknown; associates with ribosomes |
| YPR158W | −2.9 | −2.3 | −2.4 | −2.5 | Putative protein of unknown function | |
| YIL037C | PRM2 | −4.0 | −1.6 | −2.5 | −2.7 | Pheromone-regulated protein; regulated by Ste12p |
| YHR177W | −3.0 | −3.2 | −2.2 | −2.8 | Putative protein of unknown function | |
| YNL277W | MET2 | −3.0 | −3.4 | −3.2 | −3.2 | l-homoserine-O-acetyltransferase |
| YJR078W | BNA2 | −4.3 | −3.4 | −2.3 | −3.3 | Putative tryptophan 2,3-dioxygenase |
| YNL246W | VPS75 | −16.8 | −12.7 | −11.6 | −13.7 | NAP domain histone chaperone |
a
Three independent experiments (1 to 3) were done.