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Validation of vps75Δ microarray data with reverse transcription and real-time (quantitative) PCR and comparison with expression in rtt109Δ cells
| Gene | Fold change in expression in:a
|
||
|---|---|---|---|
|
vps75Δ cells
|
rtt109Δ cells (QPCR) | ||
| Microarray | QPCR | ||
| HSP30 | 17.7 | 14.2 | 1.6 |
| BSC1 | 12.4 | 7.4 | 0.2 |
| MGA1 | 10.3 | 12.9 | 1.4 |
| BNA2 | −3.3 | −2.3 | 1.3 |
| MET2 | −3.2 | −2.5 | 1.2 |
Change in expression from that in wild-type cells. QPCR, real-time (quantitative) PCR.
