FIG. 5.

The chromatin transition involves loss of DNA histone contacts. (A) Schematic representation of the HO promoter, including the positions of primers used for PCR. (B and C) DNA associated with myc-tagged H2B or the H3 C terminus (term) was enriched for by ChIP (TOH1012). The enrichment at either the URS (B) or coding region (C) was calculated relative to the HEM13 gene, which was used as a control in real-time PCR. (D) The reduction in histone contacts at the URS is dependent on Snf2, as it does not occur in a strain bearing the snf2 E834K mutation. The error bars indicate standard deviations.