FIG. 6.

Nucleosome remodeling at HO requires Asf1. Indirect end labeling was used to monitor the chromatin structure at HO 0 min and 30 min following Cdc20 arrest using the NcoIF probe as described in the legend to Fig. 1. (A) Deletion of ASF1 (TOH1045) resulted in a reduction in the disruption of the chromatin structure at 30 min in comparison to the isogenic ASF1 plus TOH1064. (B) This was also evident as a failure to lose histone contacts, as assessed by H2B ChIP. (C) Real-time RT-PCR was used to measure the levels of the HO mRNA transcript. A reduction in cell cycle-regulated transcription was observed following deletion of ASF1. The error bars indicate standard deviations.