. 2009 May 26;29(15):4103–4115. doi: 10.1128/MCB.00090-09

TABLE 1.

Partial list of RUNX-1-associated proteins identified by whole-lane MS

Protein	No. of proteins identified in the indicated expts^f					Total no. of peptides^c	Total no. of peptides in control^d
Protein	Expt 1^a	Expt 2^a	Expt 3^b	Expt 4^b	Expt 5^b	Total no. of peptides^c	Total no. of peptides in control^d
RUNX-1	67	25	9	3	19	123	0
CBF-β	32	2	2	3	3	42	1
GATA-1	1	0	2	0	0	3	1
PML	20	6	0	0	0	26	0
PRMT-1	0	0	4	3	0	7	0
Sin3A	0	0	3	0	0	3	0
FLI-1	22	0	5	3	1	31	0
Total no. of proteins	183	46	358	243	73
Total no. of specific proteins^e	152	14	219	143	22

Tandem anti-FLAG-SA affinity purification.

Single SA affinity purification.

Experiment-to-experiment variation in the number of peptides obtained for each of the proteins likely reflects differences in the amount of input material used, purification strategy, and/or sampling and detection issues related to the LC−MS-MS analysis. The total number of peptides corresponding to each protein from five independent purifications is shown.

From cells expressing birA alone.

Proteins not identified in cells expressing birA alone.

For each experiment, the total number of milligrams of input protein used was as follows: experiment 1, 288 mg; experiment 2, 120 mg; experiment 3, 170 mg; experiment 4, 55 mg; experiment 5, 100 mg.