Fig. 2.
Raman spectra obtained in a human artery grafted to the beating heart of a living swine. Spectra were processed from 16 averaged frames, representing a total acquisition time of 4 s. (a) FP and (b) HW spectra obtained at Site #1, with (c) corresponding hematoxylin and eosin histology. (d) FP and (e) HW spectra obtained at Site #2, with (f) corresponding Oil-red-O histology. Black arrows denote brown-orange staining of lipid in the direction of the spectral measurement and dark blue staining of a large calcific nodule nearby. Red arrows in (c) and (f) denote spectral measurement locations and directions. Calc.—calcium hydroxyapatite. Scale bars, 0.5 mm.