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. Author manuscript; available in PMC: 2009 Jul 26.
Published in final edited form as: Traffic. 2007 Apr;8(4):389–401. doi: 10.1111/j.1600-0854.2007.00540.x

Figure 1. Cellular entry of cationic polymers, lipids, and polypeptides is mediated by cell surface proteoglycans.

Figure 1

A) Transfection of BS-C-1 cells by PEI and LF is inhibited by sodium chlorate, a drug that inhibits proteoglycan synthesis, in a concentration dependent manner. B) Transfection by PEI and LF is much less efficient in a proteoglycan-deficient cell line of CHO cells (PGD-CHO) than in wildtype CHO cells. Transfection efficiencies are normalized against untreated cells. Based on experiments done in triplicate, the error in the transfection measurement is 10%. C) Sodium chlorate (NaClO3, 80 mM) treatment blocks the binding of PA-QDs and anti-HSPG, but not that of transferrin (Tfn), to the surface of BS-C-1 cells. The diffuse red signal in the images is due to out of focus fluorescence on the thicker regions of the cells. Scale bars: 10 μm.