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. 2009 Aug 7;5(8):e1000539. doi: 10.1371/journal.ppat.1000539

Figure 4. Genetic complementation experiments in misfit ko parasites.

Figure 4

(A) Genetic crosses of Δpbmisfit with a female gamete deficient (Δpbs47) or male gamete deficient (Δpbs48/45, Δpcdk4 and Δmap2) P. berghei mutants. Bars show mean 12-day-old oocyst numbers in midguts of A. stephensi mosquitoes. Left panel shows infections performed via membrane-feeding on ookinetes in in vitro cultures that were initiated with blood of mice co-infected with the indicated parasite mutants. The right panel shows infections of mosquitoes that directly fed on co-infected mice. Standard errors are shown; n indicates the number of midguts. (B) Microscopy observations of hybrid Δpbmisfit/Δpbs47 and Δpbmisfit/Δpbs48/45 oocysts (green) developing on A. gambiae midguts 12 days pbf. All images were obtained at 63× magnification. DIC, differential interference contrast.