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. 2008 Dec 18;41(2):136–145. doi: 10.1165/rcmb.2008-0363OC

Figure 2.

Figure 2.

XCL1 gene silencing and protein suppression. (A) siRNA targeting XCL1 induced transient gene silencing and suppression of protein expression. After one single dose (15 μg/mouse) of XCL1-targeting siRNA (open bars), nontargeting siRNA (gray bars), or PBS (closed bars), the levels of xcl1 transcripts in each mouse at 3 or 5 days after treatment were determined using quantitative RT-PCR. Mice at 3 days after treatment with XCL1-targeting siRNA had a 50% decreases in xcl1 transcripts, but, 5 days after treatment, expression levels of xcl1 transcripts were similar to those of control mice. (B) Levels of XCL1 protein. Mice receiving XCL1-targeting siRNA (open bars) at 3 and 5 days after treatment had a 40–50% reduction in the levels of XCL1 protein, respectively, when compared with similar samples obtained from control groups (closed and gray bars). Data presented represent the average (±SEM) of XCL1 mRNA (%) or XCL1 production from PBS, nontargeting siRNA, or XCL1-targeting siRNA.