Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jan 8;41(2):189–198. doi: 10.1165/rcmb.2008-0131OC

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009, American Thoracic Society

PMC Copyright notice

Figure 2. — Cigarette smoke extract (CSE) prevents respiratory syncytial virus (RSV)-induced caspase 3 and 7 activation: (A) Diagram of experimental design: four groups were exposed to either control media for 72 hours, 2% cigarette smoke extract for 72 hours, RSV (MOI 2) for 24 hours after 48 hours of exposure to control media, or 2% cigarette smoke extract for 48 hours followed by RSV (MOI 2) and cigarette smoke extract for an additional 24 hours. (B) hTBE cells were plated in an adherent monolayer and exposed to control media for 72 hours, 2% cigarette smoke extract for 72 hours, RSV (MOI 2) for 24 hours after 48 hours of exposure to control media, or 2% cigarette smoke extract for 48 hours followed by RSV (MOI 2) and cigarette smoke extract for an additional 24 hours. The cells were then harvested for whole cell protein, and Western blots for cleaved caspase 3 and cleaved caspase 7 were performed. Results shown are representative of three experiments performed on cells from three separate donors. (C) hTBE cells were plated in an adherent monolayer and exposed to control media for 72 hours, 2% cigarette smoke extract for 72 hours, staurosporine 20 μM for 24 hours after exposure to control media for 48 hours, or 2% cigarette smoke extract for 48 hours followed by staurosporine 20 μM and cigarette smoke extract for 24 hours. The cells were then harvested for whole cell protein, and Western blots for cleaved caspase 3 and 7 were performed. Results shown are representative of three experiments performed on cells from three separate donors. (D) hTBE cells were plated in an adherent monolayer and exposed to cigarette smoke extract for varying amounts of time before RSV exposure. Protein lysates were collected and Western blots for cleaved caspases 3 and 7 performed. Some reduction in caspase cleavage is evident after 24 hours of pretreatment with cigarette smoke extract, but 48 hours of cigarette smoke extract exposure before RSV infection is needed to see a marked and reliable reduction in both cleaved caspases 3 and 7.

Figure 2. — Cigarette smoke extract (CSE) prevents respiratory syncytial virus (RSV)-induced caspase 3 and 7 activation: (A) Diagram of experimental design: four groups were exposed to either control media for 72 hours, 2% cigarette smoke extract for 72 hours, RSV (MOI 2) for 24 hours after 48 hours of exposure to control media, or 2% cigarette smoke extract for 48 hours followed by RSV (MOI 2) and cigarette smoke extract for an additional 24 hours. (B) hTBE cells were plated in an adherent monolayer and exposed to control media for 72 hours, 2% cigarette smoke extract for 72 hours, RSV (MOI 2) for 24 hours after 48 hours of exposure to control media, or 2% cigarette smoke extract for 48 hours followed by RSV (MOI 2) and cigarette smoke extract for an additional 24 hours. The cells were then harvested for whole cell protein, and Western blots for cleaved caspase 3 and cleaved caspase 7 were performed. Results shown are representative of three experiments performed on cells from three separate donors. (C) hTBE cells were plated in an adherent monolayer and exposed to control media for 72 hours, 2% cigarette smoke extract for 72 hours, staurosporine 20 μM for 24 hours after exposure to control media for 48 hours, or 2% cigarette smoke extract for 48 hours followed by staurosporine 20 μM and cigarette smoke extract for 24 hours. The cells were then harvested for whole cell protein, and Western blots for cleaved caspase 3 and 7 were performed. Results shown are representative of three experiments performed on cells from three separate donors. (D) hTBE cells were plated in an adherent monolayer and exposed to cigarette smoke extract for varying amounts of time before RSV exposure. Protein lysates were collected and Western blots for cleaved caspases 3 and 7 performed. Some reduction in caspase cleavage is evident after 24 hours of pretreatment with cigarette smoke extract, but 48 hours of cigarette smoke extract exposure before RSV infection is needed to see a marked and reliable reduction in both cleaved caspases 3 and 7.