Figure 1.
Incubation of neutrophils with Toll-like receptor (TLR) 2 and TLR4 ligands induces mammalian target of rapamycin (mTOR) complex 1 (mTORC1) activation in a dose- and time-dependent manner. Bone marrow neutrophils were cultured with the indicated concentrations of Pam3 Cys-Ser-(Lys)4 (PAM) and LPS for 90 minutes (A and C) or for the indicated times with 1 μg/ml PAM or LPS (B and D). Cell extracts were then subjected to SDS-PAGE Western blot analysis with antibodies specific for total and phosphorylated ribosomal protein S6 (rpS6) (Ser240/244). Representative Western blots are shown, as well as densitometry determinations from three independent experiments (mean ± SEM; *P < 0.05 or **P < 0.01 compared with untreated).