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. Author manuscript; available in PMC: 2009 Jul 27.

Published in final edited form as: Int J Cancer. 2009 Apr 15;124(8):1980–1989. doi: 10.1002/ijc.24139

TRAF2 and RIP as modulators of NF-κB and regulators of TRAIL- and TNFα-induced apoptosis. (A) Reduction of TRAF2 and RIP protein levels correlated with sensitivity to TRAIL- and TNFα-induced apoptosis. (B) Silencing TRAF2, RIP or both reduced NF-κB activity (top panel) and partially sensitized PC3 cells to TNFα (bottom panel). Western blots were performed with TRAF2- and RIP-specific antibodies to detect the efficiency of RNAi. Non-specific siRNA was used as negative control. GAPDH was used as loading control. (C) Silencing TRAF2, RIP or both reduced NF-κB activity (top panel) and partially sensitized PC3-TR cells to TRAIL (bottom panel). Western blots were performed to detect the efficiency of RNAi. (D) Silencing TRAF2, RIP or both reduced NF-κB activity (top panel) and partially sensitized PC3-TR cells to TNFα (bottom panel). Apoptosis is measured by flow cytometric analysis of the sub-G1 population. Error bars (SD) are results of at least three independent experiments.

TRAF2 and RIP as modulators of NF-κB and regulators of TRAIL- and TNFα-induced apoptosis. (A) Reduction of TRAF2 and RIP protein levels correlated with sensitivity to TRAIL- and TNFα-induced apoptosis. (B) Silencing TRAF2, RIP or both reduced NF-κB activity (top panel) and partially sensitized PC3 cells to TNFα (bottom panel). Western blots were performed with TRAF2- and RIP-specific antibodies to detect the efficiency of RNAi. Non-specific siRNA was used as negative control. GAPDH was used as loading control. (C) Silencing TRAF2, RIP or both reduced NF-κB activity (top panel) and partially sensitized PC3-TR cells to TRAIL (bottom panel). Western blots were performed to detect the efficiency of RNAi. (D) Silencing TRAF2, RIP or both reduced NF-κB activity (top panel) and partially sensitized PC3-TR cells to TNFα (bottom panel). Apoptosis is measured by flow cytometric analysis of the sub-G1 population. Error bars (SD) are results of at least three independent experiments.