Table 1.
Primary antibodies used in this study
| Species | Antigen | Source and Catalog # | Dilution | |
|---|---|---|---|---|
| WB | IS | |||
| Chicken | P0 | Gift from Dr.Gerry Shaw, UF | 1:10000 | 1:500 |
| Rabbit | PMP22 | Pareek et. al, 1997; n/a | 1:1000 | n/a |
| Rat | MBP | Chemicon, Temecula; MAB386 | 1:5000 | n/a |
| Mouse | GAPDH | Encor Biotechnology Inc.; MCA-1D4 | 1:5000 | n/a |
| Mouse | NF-H | Encor Biotechnology Inc.; MCA-NAP4 | 1:500 | n/a |
| Chicken | NF-M | Encor Biotechnology Inc.; CPCA-NF-M | 1:5000 | n/a |
| Chicken | NF-L | Encor Biotechnology Inc.; CPCA-NF-L | 1:5000 | 1:1000 |
| Mouse | Vimentin | Sigma, St Louis, MO, USA; clone VIM 13.2 | 1:1000 | 1:500 |
| Chicken | MBP | Encor Biotechnology, Inc.; CPCA-MBP | n/a | 1:500 |
| Rabbit | p75NTR | Chemicon; AB1554 | 1:10000 | n/a |
| Mouse | pHH3 (Ser10) | Millipore, Temecula; 05–598 | 1:500 | 1:200 |
| Rabbit | Pan-Nav (α-subunit) | Chemicon; AB5210 | 1:250 | 1:100 |
| Rabbit | Kv1.1 | Chemicon; AB5174 | 1:250 | 1:100 |
| Rabbit | HSF1 | Stressgen; Victoria British Columbia; SPA- 901 |
1:500 | 1:250 |
| Rat | HSP90 | Stressgen, SPA-835 | 1:2000 | n/a |
| Rabbit | HSP70 | Stressgen; SPA-812 | 1:1000 | 1:500 |
| Rabbit | HSP40 | Stressgen; SPA-400 | 1:2000 | n/a |
| Goat | HSP27 | Santa Cruz Biotechnology, Inc.; sc-1049 | 1:2000 | n/a |
| Rabbit | αB-crystallin | Stressgen; SPA-223 | 1:1000 | n/a |
| Rabbit | LAMP1 | Gift from Dr. William Dunn Jr, UF | 1:2000 | n/a |
| Rabbit | Atg7 | Gift from Dr. William Dunn Jr, UF | 1:3000 | n/a |
| Rabbit | pS6 (Ser235/236) | Cell Signaling Technology, Inc., 2211 | 1:800 | n/a |
| Mouse | S6 | Cell Signaling Technology, Inc.; 2317 | 1:800 | n/a |
| Mouse | HSP70 | Stressgen; SPA-810 | 1:1000 | 1:250 |
| Mouse | LAMP1 | Stressgen; VAM-EN001 | n/a | 1:250 |
| Rabbit | Cathepsin-D | Cortex Biochem, San Leanardo, CA, USA; CP3090 |
1:1000 | n/a |
| Rabbit | LC3 | Cell Signaling Technology; 2775 | 1:1000 | 1:200 |
WB: Western Blot; IS: Immunostaining; n/a: non-applicable