Figure 3.
Effect of viruses on pneumococcal adherence to A549 cells. Cells were incubated for 30 min with 1 μg/ml or 0.2 μg/ml of influenza viruses, with or without the NA inhibitor oseltamivir, before the assay of adherence of R6T pneumococcus to A549 cells. Comparison of HK/Sing/PR8, which has high NA activity, with 3 representative viruses with lower NA activities is shown. Data are shown as mean ± SD of 3−6 consecutive, independent experiments. An asterisk (*) indicates a significant difference between different treatments with the same virus by ANOVA (P<0.05). A double asterisk (**) indicates a significant difference between HK/Sing/PR8 and the other viruses (0.2 μg/ml) without oseltamivir treatment by ANOVA (P<0.05).