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Determination of expression profile of ponsin splice variants in the mouse lens. Four different sequence-specific oligonucleotide primer sets (derived from the different regions of the CAP/ponsin sequence) were used to amplify ponsin-specific DNA fragments by RT-PCR analysis using total RNA derived from 1-day-old mouse lenses. The DNA fragments amplified from 30 PCR cycles are depicted. Sequence specificity was confirmed by direct sequencing.
