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. Author manuscript; available in PMC: 2009 Jul 27.
Published in final edited form as: J Neurovirol. 2008 Jan;14(1):62–72. doi: 10.1080/13550280701809084

Figure 3.

Figure 3

PDGF protects SH-SY5Y cells against gp120-mediated toxicity. A) Phase contrast microscopy of SH-SY5Y cells treated with gp120 (200ng/ml) and/or PDGF-BB (20ng/ml) for 5 days. Cells treated with gp120 alone showed rounding (as shown by black arrows) with shortened neurites. Pre-treatment of gp120-treated neurons with PDGF-BB resulted in maintenance of cellular architecture with extended neuronal processes (indicated by white arrows). Scale bars, 50 μM. Neuronal viability in the presence of gp120 and/or PDGF-BB. SH-SY5Y cells (B) and rat primary neuronal culture (C) were exposed to gp120 (200ng/ml) with or without pre-treatment with PDGF-BB (20ng/ml). Supernatant fluids and cellular extracts were collected in 24h for LDH assay. Data are presented as mean ± SEM from three independent experiments. Statistical analysis was performed using Student’s t-test. (*p < 0.01 gp120 vs control, #p<0.01 gp120 vs PDGF+gp120).