Table 1.

Synthetic DNA Template and Primers Used in LDR-SERS Experiments

template/primer	sequence for LDR (5′ 3′)
WT K-Ras template	TCC ACA AAA TGA TTC TGA ATT AGC TGT ATC GTC AAG
	GCA CTC TTG CCT ACG CCA CCA GCT CCA ACT ACC ACA AGT
	TTA TAT TCA GTC ATC
MT K-Ras template	TCC ACA AAA TGA TTC TGA ATT AGC TGT ATC GTC AAG
	GCA CTC TTG CCT ACG CCA TCA GCT CCA ACT ACC ACA AGT
	TTA TAT TCA GTC ATC
common LDR primer	5Phosa/TGG CG/AmTb/ AGG CAA GAG TGC CTT GAC
mutant LDR primer	GAA TAT AAA CTT GTG GTA G/FlurTc/T GGA GCT GA

^a5Phos denotes a 5′ phosphorylation.

^bAmT denotes an aminated thymine.

^cFlurT denotes a fluorescein dT.

The italicized bases of the template sequences are the complementary nucleotides to both primers. The 3′ base in mutant LDR primer (bold) allows for specific discrimination of the two templates.