Skip to main content
. Author manuscript; available in PMC: 2010 Jul 8.
Published in final edited form as: J Am Chem Soc. 2009 Jul 8;131(26):9321–9325. doi: 10.1021/ja9035225

Figure 1.

Figure 1

Dithionite-dependency of precursor maturation on NifEN. NifENPrecursor was first converted to NifEN“FeMoco” in varying concentrations of dithionite (0, 2, 10, 20 and 40 mM, respectively) and, subsequently, NifEN“FeMoco” was re-isolated and used as a FeMoco source for the reconstitution of FeMoco-deficient ΔnifB MoFe protein. Shown are the specific C2H2-reducing activities of MoFe protein upon reconstitution with NifEN“FeMoco” prepared at different dithionite concentrations. The calculated

solution potentials9 are indicated.