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. Author manuscript; available in PMC: 2010 Jul 8.
Published in final edited form as: J Am Chem Soc. 2009 Jul 8;131(26):9321–9325. doi: 10.1021/ja9035225

Table 1.

Reconstitution of FeMoco-deficient MoFe protein by various FeMoco sources.

Activities (nmol/mg protein/min)
FeMoco Source C2H2 formation under C2H2/Ar H2 formation under Ar NH3 formation under N2 H2 formation under N2
NifENPrecursor 0 0 0 0
Isolated FeMoco 917 ± 105 1269 ± 154 305 ± 75 292 ± 20
NifEN“FeMoco”(20mM) 1065 ± 88 1100 ± 19 334 ± 40 323 ± 23
NifEN“FeMoco”[Ti(III) citrate] 680 ± 28 890 ± 35 272 ± 32 225 ± 9
NifEN“FeMoco”(Fld_1) 538 ± 20 520 ± 21 210 ± 17 120 ± 5