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. Author manuscript; available in PMC: 2009 Dec 5.
Published in final edited form as: J Mol Biol. 2009 Apr 14;389(2):349–364. doi: 10.1016/j.jmb.2009.04.014

Fig. 2. Representative reverse-phase HPLC profiles of unphosphorylated and phosphorylated PhoB.

Fig. 2

Untreated (solid line) and phosphoramidate-treated (dashed line) wild-type PhoB (a) and PhoBD101R/R115D (b) were analyzed on a reverse-phase HPLC column as described in Materials and Methods. The steady-state phosphorylation levels attained for wild-type PhoB, PhoBD101R/R115D, PhoBR115D and PhoBF20D are listed in Table 1.