Fig. 5. Representative sedimentation equilibrium profiles of wild-type PhoB and PhoBF20D.
Wild-type PhoB (circles) and PhoBF20D (triangles) were analyzed in both their unphosphorylated (a) and phosphorylated (b) forms. Profiles are shown for the 20 μM samples that were spun at 30k RPM with the corresponding residuals of the fits shown above the profiles. For unphosphorylated and phosphorylated wild-type PhoB, as well as phosphorylated PhoBF20D, the monomer-dimer fit is shown, while for unphosphorylated PhoBF20D the monomer fit is shown.