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. Author manuscript; available in PMC: 2009 Dec 5.
Published in final edited form as: J Mol Biol. 2009 Apr 14;389(2):349–364. doi: 10.1016/j.jmb.2009.04.014

Fig. 6. Representative fluorescence anisotropy DNA-binding curves.

Fig. 6

Binding isotherms for the phosphorylated forms of wild-type PhoB (circles) and PhoBF20D (diamonds) are shown to illustrate typical fluorescence anisotropy binding curves. A synthetic oligonucleotide (Fig. S1) that contains a portion of the phoB promoter was used in these experiments. Dissociation constants for all the proteins analyzed are listed in Table 3.