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. Author manuscript; available in PMC: 2009 Dec 5.
Published in final edited form as: J Mol Biol. 2009 Apr 14;389(2):349–364. doi: 10.1016/j.jmb.2009.04.014

Fig. 7. Transcriptional activation of phoA by PhoB mutants.

Fig. 7

Cells expressing the various mutants of PhoB were grown under low phosphate (inducing) and high phosphate (non-inducing) conditions and analyzed for AP activity as described in Materials and Methods. The “&” symbol is used to identify those cells that express two mutants from different plasmids while a “/” symbol is used to designate the double-residue mutant. Each value represents a minimum of 3 replicates with error bars representing one standard deviation. The AP activity was normalized to the level attained by wild-type PhoB under low phosphate conditions.