Table 1.
Autophosphorylation of PhoB proteins using phosphoramidate as donor
| Protein | % Phosphorylation (steady-state)a | Phosphorylation Rate, kobs (s−1)b |
|---|---|---|
| Wild-type PhoB | 93 | 7.0 ±0.1 × 10−3 |
| PhoBR115D | 60 | 0.6 ±0.1 × 10−3 |
| PhoBD101R/R115D) | 92 | 3.4 ± 0.5 × 10−3 |
| PhoBF20D | 93 | 7.9 ± 0.2 × 10−3c |
| PhoBR91E/E111R | 100 | 10.1 ±0.2 × 10−3c |
a
The steady-state phosphorylation level was determined by HPLC analysis.
b
The rate of phosphorylation was measured by monitoring intrinsic protein fluorescence changes.
c
The error refers to the goodness of the fit for only one replicate, for all other kobs values the error represents one standard deviation from a minimum of two replicates.