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. Author manuscript; available in PMC: 2009 Dec 5.
Published in final edited form as: J Mol Biol. 2009 Apr 14;389(2):349–364. doi: 10.1016/j.jmb.2009.04.014

Table 3.

DNA binding activities of PhoB proteins

Protein Kd(nM), Unphosphorylateda Ka (nM), Phosphorylateda
Wild-type PhoB 145 ± 30 9.7 ± 2.2
PhoBD101R/R115D NSb 23.1 ± 6.3
PhoBR115D 256 ± 41 183 ± 18
PhoBF20D 457 ± 50 8.3 ± 1.4
PhoBR91E/E111R 86.2 ± 2.6 2.9 ± 0.3
a

The error represents one standard deviation from a minimum of three replicates.

b

Non-specific binding.