Skip to main content
. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Vaccine. 2009 Mar 3;27(18):2426–2436. doi: 10.1016/j.vaccine.2009.02.073

Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid Relevant characteristics Reference or source
Strains
F. tularensis LVS Attenuated F. tularensis vaccine strain CBER/FDA
LVSΔguaA F. tularensis LVS mutant in guaA locus This study
LVSΔguaB F. tularensis LVS mutant in guaB locus This study
E coli DH5α deoR, endA1, gyrA96, recA1, relA1, supE44 Laboratory collection
Plasmids
pUC19 Cloning vector (Ampr) Laboratory collection
pBlue-ScriptKS+ Cloning vector (Ampr) Laboratory collection
pUCGA Containing guaA5′-aphT-guaA3′ cassette This study
pBSGB Containing guaB5′-aphT-guaB3′ cassette This study
pSacB Containing the sacB gene This study
pFT695 guaA suicide plasmid This study
pFT724 guaB suicide plasmid This study
pFT906 Plasmid containing PguaB promoter This study
PFT916 Plasmid containing PguaB-guaA fragment This study
pKK214 Plasmid used to trans complement mutants UTSA
pKK214guaA pKK214 plasmid containing full-length guaA This study
pKK214guaB pKK214 plasmid containing full-length guaA This study