Table 2. Oligonucleotides used in this study.

Name	Oligonucleotide Sequences (5′ → 3′)	Combined primers and product size (bp)
Upstream guaA region
GuaA5for	CCC GGA TCC ATC TTT AGA GAT AAG TTT TCA CAT ATT GAG	GuaA5for-GuaA5rev (500)
GuaA5rev	GAT GAT ATC TTT TTA TAA AAA TAA ACA TTT TCT TAA AAG GTT ATT T
Downstream guaA region
GuaA3for	GAT GAT ATC GCT ATG TCA AAA TAT ACT ATT TTA GAT AAA ATA A	GuaA3for-GuaA3rev (500)
GuaA3rev	CCC GTC GAC GCG GCC GCA GAT CTA TAC CGC CAG CAT GAT TTA AGG CTT CA
Upstream guaB region
GuaB5for	CCC GGA TCC TAA ATA TAA GCC TAA GGC AGT GAT TGA TTT	GuaB5for-GuaB5rev (500)
GuaB5rev	GAT CCC CGA ATT CCT GCA GCC CGG GTT TTT GAT CTC CGT AAT TAA AAT CTA AAG AGT
Downstream guaB region
GuaB3for	CCC GAA TTC CTG CAG AAC CTC TTA ATT ATG ATT TTA ATA AGT TAT AAT	GuaB3for-GuaB3rev (500)
GuaB3rev	CCC GTC GAC GCG GCC GCA ACA ACA CCG CTT ACA GCA AAC TTT TTA
Km resistance cassette (aphT)
AphTfor	ATG CCC GGG GAA GTT CCT ATA CTT TCT AGA GAA TAG	AphTfor-AphTrev (1438)
AphTrev	ATG CCC GGG AGT TCC TAT TCC GAA GTT CCT ATT CT
GroEL promoter (PgroEL)
GroELfor	GAA TTC CGG ATC CTT TCT TGA AAA TTT TTT TTT TGA CTC AAT AT	GroELfor-GroELrev (132)
GroELrev	GAA TTC CCC CGG GCA TAT GAA TCT TAC TCC TTT GTT AAA TTA
Genotypic identification of LVS guaA mutant
OLIGA1	GGA TAA AAT AAC CTT TTA AGA AAA TGT TT	OLIGA1-OLIGA2 (565)
OLIGA2	GTT TCT GGG TGA AAC TGC ACA CC	OLIGA1-OLIGkm (855)
Genotypic identification of LVS guaB mutant
OLIGB1	ACT CTT TAG ATT TTA ATT ACG GAG ATC	OLIGB1-OLIGB2 (550)
OLIGB2	TCT TTT TAA TCG CTC CTT GAG AAG C	OLIGB1-OLIGkm (840)
aphT oligonucleotide
OLIGkm	ATG CAG CCG CCG CAT TGC ATC A
Trans complementation of guaA gene
CompA1	AGC TAG CTC CCG GGA TGA CAG ATA TAC ATA ATC ATA AGA TTT TGA TT	CompA1-CompA2 (1550)
CompA2	AGC TAG CTG ATA TCG TCG ACT TAT TCC CAT TCA ATT GTT CCA GGT GGT T
Trans complementation of guaB gene
CompB1	GGG ACT AGT CTA GAG CGA AAT AGT AAA TTC TAA TAC TTA TTT C	CompB1-ComB2 (1570)
CompB2	GGG ACT AGT CTA GAT TAA GAC TGG TAA TTA GG TGG TTC TTT AG