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. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Vaccine. 2009 Mar 3;27(18):2426–2436. doi: 10.1016/j.vaccine.2009.02.073

Table 4. Growth of F. tularensis in organs of naïve BALB/c mice.

Group (No. mice) F. tularensis straina Mean ± SD CFU of Francisella (log 10) / organ
Spleen Lung Liver Blood (ml)
1 (n=3) LVSΔguaA BDLb BDL BDL BDL
2 (n=3) LVSΔguaB 1.03 ± 0.91 (2/3)c BDL BDL BDL
3 (n=3) LVS 4.31 ± 0.76 2.82 ± 1.18 4.48 ± 0.91 2.17 ± 0.96
4 (n=3) LVSΔguaA BDL BDL BDL BDL
5 (n=3) LVSΔguaB BDL BDL BDL BDL
6 (n=4) LVS 5.91 ± 0.78 3.82 ± 1.69 5.87 ± 0.90 >2.00 d
7 (n=3) LVSΔguaA BDL BDL BDL BDL
8 (n=4) LVSΔguaB BDL BDL BDL BDL
9 (n=4) LVS 6.77 ± 0.95 4.20 ± 0.95 6.92 ± 1.09 >2.00
a

Groups 3 to 4 BALB/c mice were challenged with LVS derivatives in 1 ml of gelatin 1%-PBS. Groups 1, 4 and 7 were inoculated with 100 CFU of F. tularensis LVSΔguaA; Groups 2, 5 and 8 received 720 CFU of LVSΔguaB and groups 3, 6 and 9 were inoculated with 860 CFU of LVS by the intraperitoneal route. To determine bacterial burdens in lung, spleen and liver, inoculated mice were sacrificed on day 1 (groups: 1, 2, and 3), day 2 (groups: 4, 5 and 6) and day 3 of infection (groups: 7, 8 and 9). Tissue homogenates were serially diluted in PBS and plated on MHA-B plates, and the number of CFU per organ was calculated.

b

BDL, below detection limit (∼ 20 organisms/organ)

c

Bacteria only detected in two out of three organs

d

Bacteria detected in the organs is over 100 CFU/ml of blood