Figure 2.

SCD1 inhibition prevents diet-induced obesity and insulin resistance in LDLr^-/- Apob^100/100 mice. Starting at six weeks of age, mice were fed diets enriched in 0.1% (w/w) cholesterol and either saturated fatty acids (SFA) or monounsaturated fatty acids (MUFA) for a period up to 20 weeks in conjunction with biweekly injections (25 mg/kg) of either saline (□ in bar graphs, in line graphs), a non-targeting control ASO (■ in bar graphs, in line graphs), or SCD1 ASO (in bar graphs, in line graphs). (A) Photographs, (B) body weights, and (C) epididymal fat pad mass of mice following 20 weeks of diet and ASO treatment. Data in panels (B) and (C) represent the mean ± SEM from 8-15 mice per group, and values in panel (B) not sharing a common superscript differ significantly (p<0.05). (D) Food intake was measured after 8 weeks of diet and ASO treatment, and no significant differences were detected. (E) Fasting plasma insulin levels were measured after 4 weeks of diet and ASO treatment. Data in panel E represent the mean ± SEM from 5 mice per group, and values not sharing a common superscript differ significantly (p<0.05). (F) Glucose tolerance tests (GTT) and (G) insulin tolerance tests (ITT) were performed following 16 weeks of diet and ASO treatment. Data shown in panels (F) and (G) represent the mean ± SEM from 5 mice per group, * = significantly different than the Control ASO group within each diet group (p<0.05).