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. Author manuscript; available in PMC: 2009 Sep 30.

Published in final edited form as: Circulation. 2008 Sep 15;118(14):1467–1475. doi: 10.1161/CIRCULATIONAHA.108.793182

Effects of SCD1 inhibition on plasma lipids in LDLr ^-/-, apoB100-only mice. Starting at six weeks of age, mice were fed diets enriched in 0.1% (w/w) cholesterol and either saturated fatty acids or monounsaturated fatty acids (MUFA) for a period up to 20 weeks in conjunction with biweekly injections (25 mg/kg) of either saline (□ in bar graphs, in line graphs), a non-targeting control ASO (■ in bar graphs, in line graphs), or SCD1 ASO (in bar graphs, in line graphs). Plasma samples were collected at baseline (6 weeks of age), and after 4, 8, or 20 weeks of diet and ASO treatment. Plasma triglycerides (A) and total plasma cholesterol (B) were measured enzymatically. Data shown in panels (A) and (B) represents the mean ± SEM from 5-8 mice per group, ** = significantly different than the Control ASO group within each diet group (p<0.01). Panel (C) represents the lipoprotein cholesterol distribution of pooled plasma samples (n=5 mice per pool) from mice fed a saturated diet and treated with ASO for 20 weeks. Panel (D) represents cholesterol levels in very-low-density lipoproteins (VLDLc), low-density lipoproteins (LDLc), and high-density lipoproteins (HDLc) in mice fed a saturated diet and treated with ASO for 20 weeks. Data shown in panel (D) represents the mean ± SEM from 6 mice per group, and values not sharing a common superscript differ significantly (p<0.05). (E) Fatty acid (FA) composition (% of total FA as SFA or MUFA) of LDL and HDL cholesteryl esters (LDL-CE and HDL-CE). Data shown in panel (E) represents the mean ± SEM (n=5 per group), and values not sharing a common superscript differ significantly (p<0.05). (F) Lipoprotein size was determined by dynamic light scattering, and is represented as the mean ± SEM from 5 mice fed a saturated diet, and values not sharing a common superscript differ significantly (p<0.05). (G) Western blot analysis of whole plasma from mice fed a saturated diet for 20 weeks; antibodies used were targeting apolipoproteins B (apoB), E (apoE), AI (apoAI), and lecithin:cholesterol acyltransferase (LCAT).

Inline graphic — Effects of SCD1 inhibition on plasma lipids in LDLr ^-/-, apoB100-only mice. Starting at six weeks of age, mice were fed diets enriched in 0.1% (w/w) cholesterol and either saturated fatty acids or monounsaturated fatty acids (MUFA) for a period up to 20 weeks in conjunction with biweekly injections (25 mg/kg) of either saline (□ in bar graphs, in line graphs), a non-targeting control ASO (■ in bar graphs, in line graphs), or SCD1 ASO (in bar graphs, in line graphs). Plasma samples were collected at baseline (6 weeks of age), and after 4, 8, or 20 weeks of diet and ASO treatment. Plasma triglycerides (A) and total plasma cholesterol (B) were measured enzymatically. Data shown in panels (A) and (B) represents the mean ± SEM from 5-8 mice per group, ** = significantly different than the Control ASO group within each diet group (p<0.01). Panel (C) represents the lipoprotein cholesterol distribution of pooled plasma samples (n=5 mice per pool) from mice fed a saturated diet and treated with ASO for 20 weeks. Panel (D) represents cholesterol levels in very-low-density lipoproteins (VLDLc), low-density lipoproteins (LDLc), and high-density lipoproteins (HDLc) in mice fed a saturated diet and treated with ASO for 20 weeks. Data shown in panel (D) represents the mean ± SEM from 6 mice per group, and values not sharing a common superscript differ significantly (p<0.05). (E) Fatty acid (FA) composition (% of total FA as SFA or MUFA) of LDL and HDL cholesteryl esters (LDL-CE and HDL-CE). Data shown in panel (E) represents the mean ± SEM (n=5 per group), and values not sharing a common superscript differ significantly (p<0.05). (F) Lipoprotein size was determined by dynamic light scattering, and is represented as the mean ± SEM from 5 mice fed a saturated diet, and values not sharing a common superscript differ significantly (p<0.05). (G) Western blot analysis of whole plasma from mice fed a saturated diet for 20 weeks; antibodies used were targeting apolipoproteins B (apoB), E (apoE), AI (apoAI), and lecithin:cholesterol acyltransferase (LCAT).