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. Author manuscript; available in PMC: 2009 Nov 26.

Published in final edited form as: Circulation. 2009 May 11;119(20):2708–2717. doi: 10.1161/CIRCULATIONAHA.108.823740

A and B: Representative scatter pattern (cell size [FSC] versus granularity [SSC]) of total blood leukocytes and some phenotypic characteristics of blood CD11c⁺ cells from WT and apoE^-/- mice on HFD analyzed by FACS. C: Relative ratio of CD11c⁺ to CD11c^- monocytes in apoE^-/- mice on HFD (R1/granulocyte and R2/monocyte regions) or WT, n=9-12 mice/group; *P<0.001 vs. WT, #P<0.001 vs. apoE^-/- R2. D and E: CD11c⁺ monocytes in R1 from apoE^-/- mice on HFD had higher levels of CD11b and CD29 than CD11c⁺ monocytes in R2; n=5 for each of WT and apoE^-/- on HFD. F and G: Representative images of CD11c⁺ cells isolated from blood of WT or apoE^-/- on HFD by magnetic separation (F), and CD11c⁺ and CD11c^- monocytes isolated from R1 and R2 regions of apoE^-/- on HFD by cell sorting (G). Original magnification: ×600 (Neat Stain), ×100 (Oil Red O).

A and B: Representative scatter pattern (cell size [FSC] versus granularity [SSC]) of total blood leukocytes and some phenotypic characteristics of blood CD11c⁺ cells from WT and apoE^-/- mice on HFD analyzed by FACS. C: Relative ratio of CD11c⁺ to CD11c^- monocytes in apoE^-/- mice on HFD (R1/granulocyte and R2/monocyte regions) or WT, n=9-12 mice/group; *P<0.001 vs. WT, #P<0.001 vs. apoE^-/- R2. D and E: CD11c⁺ monocytes in R1 from apoE^-/- mice on HFD had higher levels of CD11b and CD29 than CD11c⁺ monocytes in R2; n=5 for each of WT and apoE^-/- on HFD. F and G: Representative images of CD11c⁺ cells isolated from blood of WT or apoE^-/- on HFD by magnetic separation (F), and CD11c⁺ and CD11c^- monocytes isolated from R1 and R2 regions of apoE^-/- on HFD by cell sorting (G). Original magnification: ×600 (Neat Stain), ×100 (Oil Red O).