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. Author manuscript; available in PMC: 2009 Jul 27.
Published in final edited form as: Mol Ther. 2008 Jun 24;16(9):1594–1601. doi: 10.1038/mt.2008.136

Figure 1. Schematics of viral constructs for double oxygen–sensing vector system.

Figure 1

Six recombinant adenoviruses were generated for the double oxygen–sensing virus system. The four sensor vectors (top) have the GAL4-ODD-p65-coding sequence driven by different promoters: cytomegalovirus (CMV), Myh6, 2xHRE-mp, or 6xHRE-mp. Derivation of components: GAL4-yeast DNA-binding domain, ODD-oxygen-dependent degradation domain of hypoxia-inducible factor-1α, p65/RelA-the human pol II–activated domain from nuclear factor κB. The two effector vectors (bottom) consisted of six UAS-GAL4 binding sites upstream of a minimal viral promoter (E1BTATA) that was able to amplify the hypoxia signal by binding GAL4-ODD-p65 protein to activate transcription of enhanced green fluorescent protein (eGFP) or luciferase reporter genes. HRE, hypoxia-responsive element; LTR, left-terminal repeat; RTR, right-terminal repeat.