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. Author manuscript; available in PMC: 2009 Jul 27.
Published in final edited form as: Mol Ther. 2008 Jun 24;16(9):1594–1601. doi: 10.1038/mt.2008.136

Figure 2. Hypoxia-mediated amplification of transcriptional activity in double oxygen–sensing vector system (DOSVS).

Figure 2

Rat cardiac myocytes were transduced with one of four different sensor viruses (Figure 1) containing unique promoters: 2xHRE-mp, 6xHRE-mp, Myh6, and cytomegalovirus (CMV) fused with GAL4-ODD-p65. The same myocytes were also transduced with the 6UAS-luciferase effector virus. The CMV-luciferase virus was used as a positive control of maximal adenoviral gene transfer efficiency. (a) At 48 hours after transduction, promoter activity of each pair of viruses was assessed by luciferase assay. (b) The transcriptional activity of each set of DOSVS was also normalized to CMV-luciferase activity. In each graph, “+” is hypoxic and “−” is normoxic. Values are mean ± SEM. HRE, hypoxia-responsive element; NS, not significant; ODD, oxygen-dependent degradation domain.