Figure 2).

Effect of angiotensin II (Ang II) on NADH dehydrogenase subunit 5 (ND5) content. Neonatal cardiomyocytes were incubated for three days in control medium. On the third day, some cells were treated for 6 h with medium containing either 1 nM Ang II, or 1 nM Ang II and the mitochondrial permeability transition pore inhibitor, cyclosporin A (CsA, 200 nM). Following treatment, the cardiomyocytes were harvested, mitochondria isolated and Western blots of ND5 obtained. Shown above is a representative Western blot, with the means ± SEM of five different preparations shown below. *A significant difference between the Ang II-treated and control cells (P<0.05); ^#A significant difference between the CsA plus Ang II and the Ang II groups (P<0.05)