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. Author manuscript; available in PMC: 2009 Jul 27.
Published in final edited form as: J Neuroimmunol. 2007 Feb 23;185(1-2):75–86. doi: 10.1016/j.jneuroim.2007.01.012

Figure 3.

Figure 3

IL-12 treatment increases IFN-γ, PD-L1, and PD-L2 gene expression in spleen of EAE mice. Total RNA was extracted from spleen in C57BL/6 EAE mice treated with rmIL-12 or PBS (n = 5 mice/group) on day 7 p.i., and 5 μg RNA was used to generated cDNA. Quantitative RT-PCR was performed. β-actin was amplified in each sample as endogenous control. Each sample was run in triplicate. Relative quantization was used to calculate the results of the quantization assays with the comparative CT methods. Quantization of gene expression was reported as the fold difference relative to the housekeeping gene. Data were analyzed using GeneScan software. Electrophoresis of final products of PCR of IFN-γ, PD-1, PD-L1, PD-L2 gene and β-actin gene as endogenous control in 2.5% agarose-TBE gel is shown (A). There is a significant difference of IFN-γ gene expression between the PBS and rmIL-12-treated groups (** p<0.01) (B). Compared with the PBS group, PD-L1 and PD-L2 gene expression were increased significantly in the rmIL-12 treated group (** p<0.01, * p<0.05), and there is an increasing tendency of PD-1 gene expression but no significant difference (p>0.05) (C). One representative experiment of three is shown.