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. Author manuscript; available in PMC: 2009 Jul 27.
Published in final edited form as: J Neuroimmunol. 2007 Feb 23;185(1-2):75–86. doi: 10.1016/j.jneuroim.2007.01.012

Figure 4.

Figure 4

IL-12 treatment induces MOG-specific T cells to produce IFN-γ in vitro. MOG-specific T cells isolated from inguinal and popliteal lymph nodes were prepared on day 10 p.i. from C57BL/6 mice immunized with 300 μg MOG35-55 peptide in CFA. MOG-specific T cells were cultured without any antigen as control (no Ag), or with MOG35-55 stimulation alone at a final concentration of 25 μg/ml (MOG), or with MOG stimulation and co-cultured with 20 ng/ml rmIL-12 (MOG + IL-12). Supernatant was harvested after 48 hours for determining the production of cytokines by Flow Cytometry using the Mouse Th1/Th2 Cytokine Cytometric Bead Array Kit. Results of cytokine production were expressed as concentration in supernatant. Data represent mean of triplicate values. Compared with the “no Ag” group, production of IL-2, TNF-α and IFN-γ in the “MOG” group were significantly increased (## p<0.01, # p<0.05), but production of IL-4 and IL-5 did not change (p>0.05). Compared with the “MOG” group, production of IFN-γ in the “MOG + IL-12” group was significantly increased (** p<0.01), but production of IL-2, IL-4, IL-5 and TNF-α did not change (p>0.05). The figure is a representative of three experiments.