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. Author manuscript; available in PMC: 2009 Jul 27.
Published in final edited form as: J Neuroimmunol. 2007 Feb 23;185(1-2):75–86. doi: 10.1016/j.jneuroim.2007.01.012

Figure 6.

Figure 6

IL-12 treatment does not increase PD-L gene expression in spleen of IFN-γ-deficient EAE mice. Total RNA was extracted from spleen of IFN-γ-deficient EAE mice treated with rmIL-12 or PBS on day 7 p.i., and 5 μg RNA was used to generated cDNA. Quantitative RT-PCR was performed. β-actin was amplified in each sample as endogenous control. Each sample is triplicate. Quantization of gene expression was reported as the fold difference relative to the housekeeping gene. Data were analyzed using GeneScan software. Electrophoresis of final products of PCR of PD-1, PD-L1, PD-L2 gene and β-actin as endogenous control in 2.5% agarose-TBE gel is shown (A). There is no significant difference in PD-1, PD-L1 and PD-L2 gene expression between the PBS and rmIL-12-treated groups (p>0.05) (B). One representative experiment of three is shown.