Figure 1.

Chemomodulation of phenotype of human DCs by antineoplastic chemotherapeutic agents in low noncytotoxic concentrations. DCs were generated from monocyte isolated from PBMC of healthy volunteers by culturing monocytes in complete medium supplemented with GM-CSF and IL-4 as described in Materials and Methods. Chemotherapeutic agents were added to DC cultures for 48 h and DCs were harvested on day 6 for phenotypic analysis. Results of a representative experiment assessing the co-expression of CD83 and HLA-DR (A) or CD40 (B) on control and drug-treated DCs are shown. Similar data were obtained in three independent experiments using PBMC from three different donors. Control, non-treated DCs.