Figure 2.

Up-regulation of antigen-presenting function of human DCs treated with chemotherapeutic agents in low noncytotoxic concentrations. Human monocyte-derived DCs were treated with low nontoxic concentrations of selected drugs for 48 h. Cells were collected on day 6 and co-cultured with allogeneic nylon-wool purified T lymphocytes for 96 h. Cell cultures were pulsed with ³H-thymidine for 4 h prior to harvesting and counting in a liquid scintillation counter. The drugs were used in the following concentrations: vinblastine and vincristine, 1 nM; paclitaxel, azadeoxycytidine, and methotrexate, 5 nM; doxorubicin, 10 nM; mitomycin C, 50 nM. The mean ± SEM. *, p < 0.05 (ANOVA, N = 4). Control, non-treated DCs.